Fig. 6: ZIC5 promotes enzalutamide resistance in PCa cells.
From: AR-regulated ZIC5 contributes to the aggressiveness of prostate cancer
A RT-qPCR analysis of AR target genes (PSA, TMPRSS2), AR, and AR-V7 in 22RV1 cells transfected with sh-ZIC5 or oe-ZIC5 for 48 h. *P < 0.05 vs. control vector or sh-NC, N.S. P > 0.05 vs. control vector. B Western blot analysis of AR and AR-V7 levels in LNCAP, C4-2B, and 22RV1 cells transfected with sh-ZIC5 or sh-NC for 72 h. C, D Results of CCK8 cell proliferation assays conducted on (C) 22RV1 cells transfected with sh-ZIC5 or NC shRNA and treated with various concentrations of enzalutamide (ENZ) for 72 h, and on (D) 22RV1 cells transfected with sh-ZIC5 or NC shRNA and treated with ENZ (20 μmol/L) for 24, 48, 72 or 96 h. *P < 0.05 vs. NC. E, F Results of CCK8 cell proliferation assays conducted on (E) C4-2B cells transfected with oe-ZIC5 or vector plasmids and treated with various concentrations of ENZ for 72 h, and on (F) C4-2B cells transfected with oe-ZIC5 or vector plasmids and treated with ENZ (20 μmol/L) for 24, 48, 72 or 96 h. *P < 0.05 vs. control vector. G Flow cytometry analysis of apoptosis in C4-2B cells treated with ENZ (20 μmol/L) for 72 h and transfected, as indicated, with oe-ZIC5, si-AR. The bar graph shows quantification data from three independent experiments. *P < 0.05 vs. NC, #P < 0.05 vs. ENZ, &P < 0.05 vs. ENZ + ZIC5. H Flow cytometry analysis of apoptosis in 22RV1 cells treated with ENZ (20 μmol/L) for 72 h and transfected, as indicated, with sh-ZIC5, AR expression plasmids. The bar graph shows quantification data from three independent experiments. N.S., P > 0.05 vs. NC, #P < 0.05 vs. ENZ, &P < 0.05 vs. ENZ + sh-ZIC5.
