Fig. 1: Infection by AAV-eEF1A1 or AAV-eEF1A2 increases the level of eEF1A1 and eEF1A2 proteins, respectively, in CST neurons. | Cell Death Discovery

Fig. 1: Infection by AAV-eEF1A1 or AAV-eEF1A2 increases the level of eEF1A1 and eEF1A2 proteins, respectively, in CST neurons.

From: Overexpressing eukaryotic elongation factor 1 alpha (eEF1A) proteins to promote corticospinal axon repair after injury

Fig. 1

Representative images of eEF1A1, GFP (viruses), and NeuN staining at the layer V of the right sensorimotor cortex of injured eEF1A1-overexpressing (OE) mice (A–A”’); and injured control mice (AAV-GFP injection) (B–B”’). Representative images of eEF1A2, GFP (viruses), and NeuN staining at the layer V of the right sensorimotor cortex of injured eEF1A2-OE mice (C–C”’); and injured control mice (AAV-GFP injection) (D–D”’). E, E’ Detailed images of eEF1A1 (E), eEF1A2 (E’) and GFP (viruses) staining in CST neurons of injured eEF1As OE mice. F Quantification of eEF1A1 immunoreactivity in eEF1A1 OE and control mice. G Quantification of eEF1A2 immunoreactivity in eEF1A2 OE and control mice. H Quantification of both eEF1A1 and eEF1A2 immunoreactivity in eEF1As OE mice. Scale bars: 50 μm (A–D”’), 25 μm (E–E’). Control, 12 mice; eEF1A1, 10 mice; eEF1A2, 10 mice; eEF1As OE 8 mice. 20 cells quantified per mouse. Stats: Mann–Whitney U test. Bars show mean ± SEM. ****p < 0.0001. Mann–Whitney U test revealed no significant differences between eEF1A1 and eEF1A2 intensities in eEF1As OE animals.

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