Fig. 5: Only eEF1A2 OE mice display actin rearrangement. | Cell Death Discovery

Fig. 5: Only eEF1A2 OE mice display actin rearrangement.

From: Overexpressing eukaryotic elongation factor 1 alpha (eEF1A) proteins to promote corticospinal axon repair after injury

Fig. 5

Left panels: Representative 10× images of β-actin and GFP (viruses) staining at the right sensorimotor cortex of in control (A) and eEF1A2 OE (B) mice. Right and bottom panels: Detail from the cortical layer V (yellow box) at 20× magnification. Please note the bundled pattern of β-actin staining defining the cellular shape in NeuN+ cells (arrowheads), GFP-/GFAP+ cells (thick arrows), and GFP-/GFAP-/NeuN- cells (thin arrows). Scale bars = 50 µm. C Quantification of β-actin immunoreactivity in cortical layer V. 3 mice per genetic condition. Stats: Ordinary one-way ANOVA. Bars show mean ± SEM. **p = 0.0054, Control vs eEF1A2 OE; **p = 0.0018, eEF1A1 OE vs eEF1A2 OE.

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