Fig. 1: The neuroprotective effect of Ret (30 µM) and XE-991 (300 nM–10 µM) against GA-induced cell damage in RA-differentiated SH-SY5Y cells. | Cell Death Discovery

Fig. 1: The neuroprotective effect of Ret (30 µM) and XE-991 (300 nM–10 µM) against GA-induced cell damage in RA-differentiated SH-SY5Y cells.

From: A new K+channel-independent mechanism is involved in the antioxidant effect of XE-991 in an in vitro model of glucose metabolism impairment: implications for Alzheimer’s disease

Fig. 1

A, B, D Effect of Ret and XE-991 on cell viability, assessed by MTT assay, and C, E mitochondrial ROS production assessed by measuring MitoTracker Red CM-H2XRos fluorescence intensity in cells challenged with GA. Cells were pretreated with Ret (30 µM) and XE-991 (300 nM–10 µM) for 1 h and then exposed to GA (1 mM) for 24 h (without withdrawing Ret and XE-991). In each experiment MTT reduction and the fluorescence intensity of MitoTracker Red CM-H2XRos were expressed as control percentages. Differences among means were evaluated by one-way ANOVA followed by Dunnett’s post hoc test. A F (4, 35) = 32.35. Each column depicts the mean ± S.E.M. of at least four independent experiments that were performed in triplicate. *Significant versus all groups (p < 0.0001 versus control groups, p < 0.001 versus Ret + XE-991 + GA, p < 0.01 versus Ret + GA); **significant versus control groups (p < 0.0001) and GA (p < 0.01); #significant versus Ctl (p < 0.0001), Ret and GA (p < 0.001). B F (3, 18) = 15.52. Each column depicts the mean ± S.E.M. of at least four independent experiments that were performed in triplicate. *Significant versus all groups (p < 0.0001 versus control groups, p < 0.01 versus XE-991 + GA); **significant versus XE-991 (p < 0.05) and GA (p < 0.01). C F (6, 30) = 8.198. The bar plot shows the mean ± S.E.M. of fluorescence increase evoked by ROS production. For each experimental group, the statistical analysis was performed by using the basal values derived from at least three independent experiments, and 100–150 cells were recorded for each session. *Significant versus all groups (p < 0.0001 versus Ctl, p < 0.001 versus Ret and XE-991, p < 0.05 versus Ret + GA, XE-991 + GA and Ret + XE-991 + GA); **significant versus Ctl and GA (p < 0.05). D F (2, 21) = 27.57. Each column shows the mean ± S.E.M. of eight independent experiments that were performed in triplicate. *Significant versus all groups (p < 0.0001 versus Ctl and p < 0.05 versus XE-991 + GA); **significant versus all groups (p < 0.001 versus Ctl and p < 0.05 versus GA). E F (2, 12) = 15.92. The bar plot shows the mean ± S.E.M. of the fluorescence increase evoked by ROS production. For each experimental group, the statistical analysis was performed by using the basal values derived from five independent experiments, and 100–150 cells were recorded for each session. *Significant versus all groups (p < 0.001 versus Ctl and p < 0.05 versus XE-991 + GA); **significant versus all groups (p < 0.05). Ctl control, GA glyceraldehyde, Ret retigabine.

Back to article page