Fig. 2: The neuroprotective effect of XE-991 (300 nM) on cell viability, mitochondrial ROS production, and SOD activity measured in primary rat cortical neurons. | Cell Death Discovery

Fig. 2: The neuroprotective effect of XE-991 (300 nM) on cell viability, mitochondrial ROS production, and SOD activity measured in primary rat cortical neurons.

From: A new K+channel-independent mechanism is involved in the antioxidant effect of XE-991 in an in vitro model of glucose metabolism impairment: implications for Alzheimer’s disease

Fig. 2

A Evaluation of the effect of 1 h exposure to XE-991 (300 nM) on mitochondrial activity, B, C mitochondrial ROS production and D) SOD activity. XE-991 (300 nM) was added 1 h before GA (1 mM) exposure and maintained for the whole GA treatment (24 h). In each experiment MTT reduction, the fluorescence intensity of MitoTracker Red CM-H2XRos and SOD activity were expressed as control percentages. Differences among means were evaluated by one-way ANOVA followed by Dunnett’s post hoc test. A F (3, 23) = 32.45. Each column depicts the mean ± S.E.M. of at least three independent experiments that were performed in triplicate. *Significant versus all groups (p < 0.0001 versus control groups and p < 0.05 versus XE-991 + GA); **significant versus all groups (p < 0.001 versus Ctl, p < 0.0001 versus XE-991 and p < 0.05 versus GA). B F (3, 11) = 19.02. The bar plot shows the mean ± S.E.M. of the fluorescence increase evoked by ROS production. For each experimental group, the statistical analysis was performed by using the basal values derived from at least three independent experiments, and 100–150 cells were recorded for each session. *Significant versus all groups (p < 0.0001). C Representative pictures of mitochondrial ROS obtained by MitoTracker Red CM-H2XRos staining deriving from at least three independent experiments. Scale bar = 50 µm. D F (3, 18) = 8.763. Each column shows the mean ± S.E.M. of at least four independent experiments that were performed in triplicate. *Significant versus all groups (p < 0.01 versus Ctl, p < 0.05 versus XE-991, and p < 0.001 versus XE-991 + GA).

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