Fig. 5: Aβ expression in primary rat cortical neurons and RA-differentiated SH-SY5Y cells treated with GA and exposed to XE-991. | Cell Death Discovery

Fig. 5: Aβ expression in primary rat cortical neurons and RA-differentiated SH-SY5Y cells treated with GA and exposed to XE-991.

From: A new K+channel-independent mechanism is involved in the antioxidant effect of XE-991 in an in vitro model of glucose metabolism impairment: implications for Alzheimer’s disease

Fig. 5

A, C, E Quantification and B, D, F representative pictures of Aβ expression. The protein Aβ was detected by immunofluorescence staining (A–D) and western blot (E, F). F Immunoblotting analysis using the 6E10 antibody showed various sizes of bands corresponding to Aβ aggregates/oligomers (Aβ tetramers (Aβ4mer) ~17, Aβ oligomers (Aβo) ~25, ~40, and >~50 kDa). ANT was used as loading control to verify mitochondrial isolation, and GAPDH was used as cytoplasmic marker. In each experiment both the intensity of fluorescence and normalized optical density values of Aβ were reported as control percentages. Scale bar 50 µM. Differences among means were evaluated by one-way ANOVA followed by Dunnett’s post hoc test. A F (2, 6) = 12.53. Each column depicts the mean ± S.E.M. of three independent experiments (50–100 cells for each experimental group were analyzed). *Significant versus all groups (p < 0.01 versus Ctl, p < 0.05 versus XE-991 + GA). C F (2, 15) = 21.49. Each column depicts the mean ± S.E.M. of six independent experiments (for each experimental group, 50–100 cells were analyzed). *Significant versus Ctl (p < 0.0001) and XE-991 + GA (p < 0.05); **significant versus Ctl (p < 0.01) and GA (p < 0.05). E F (2, 9) = 14.99. Each column depicts the mean ± S.E.M. of four independent experiments. *Significant versus all groups (p < 0.01).

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