Fig. 7: Effect of XE-991 on AMPK/mTOR pathway in primary rat cortical neurons and RA differentiatedSH-SY5Y cells challenged with GA. | Cell Death Discovery

Fig. 7: Effect of XE-991 on AMPK/mTOR pathway in primary rat cortical neurons and RA differentiatedSH-SY5Y cells challenged with GA.

From: A new K+channel-independent mechanism is involved in the antioxidant effect of XE-991 in an in vitro model of glucose metabolism impairment: implications for Alzheimer’s disease

Fig. 7

Quantification of (A, C) p-AMPKα and (B, D) p-mTOR expression in primary rat cortical neurons (A, B) and RA-differentiated SH-SY5Y (C, D) treated with GA and exposed to XE-991. As loading control, β-actin was used. Normalized optical density values were reported as control percentages. Differences among means were evaluated by one-way ANOVA followed by Dunnett’s post hoc test. A F (3, 12) = 11.47. Each column depicts the mean ± S.E.M. of four independent experiments. *Significant versus all groups (p < 0.001 versus Ctl; p < 0.01 versus XE-991 and XE-991 + GA). B F (3, 12) = 4.096. Each column depicts the mean ± S.E.M. of four independent experiments. *Significant versus all groups (p < 0.05). C F (3, 11) = 5.592. Each column depicts the mean ± S.E.M. of at least three independent experiments. *Significant versus all groups (p < 0.05). D F (3, 12) = 4.903. Each column depicts the mean ± S.E.M. of at least three independent experiments. *Significant versus all groups (p < 0.05).

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