Fig. 2: Accumulation of 24S-OHC esters induced SG formation. | Cell Death Discovery

Fig. 2: Accumulation of 24S-OHC esters induced SG formation.

From: Integrated stress response is involved in the 24(S)-hydroxycholesterol-induced unconventional cell death mechanism

Fig. 2

a, b Cells grown on cover slips were pretreated for 15 min with 5 μM F12511 and then exposed for 3 h to 50 μM 24S-OHC or 1 μM Thapsi. Cells were subjected to immunofluorescence staining for TIA1 and G3BP1. a Representative confocal images (white arrows indicate colocalization). Length of scale bar = 20 μm. b Percentage of cells containing G3BP1-positive SGs. **P < 0.01; n.s. not significant. c Cells were treated as in Fig. 2a. Immunoblot analysis of TIA1, G3BP1, and β-actin. d, e Cells grown on cover slips were treated with 50 μM 24S-OHC or 1 μM Thapsi for 3 h in presence or absence of 100 μM cycloheximide (CHX). Cells were subjected to immunofluorescence staining for TIA1 and G3BP1. d Representative confocal images. e Percentage of cells containing G3BP1-positive SGs.

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