Fig. 3: 24S-OHC-induced SG formation occurred concurrently with 24S-OHC-induced protein synthesis repression. | Cell Death Discovery

Fig. 3: 24S-OHC-induced SG formation occurred concurrently with 24S-OHC-induced protein synthesis repression.

From: Integrated stress response is involved in the 24(S)-hydroxycholesterol-induced unconventional cell death mechanism

Fig. 3

a, b Cells grown on cover slips were treated with 50 μM 24S-OHC for indicated periods. Cells were subjected to immunofluorescence staining for TIA1 and G3BP1. a Representative confocal images. b Percentage of cells containing G3BP1-positive SGs. **P < 0.01; compared with cells treated with the vehicle. c Cells were treated with 50 μM 24S-OHC for indicated periods or 3 μM Thapsi for 3 h. Cells were then incubated with 10 μg/ml puromycin for final 15 min. Whole-cell lysates were subjected to immunoblotting using appropriate antibodies, as indicated.

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