Fig. 4: Inhibition of PERK axis suppressed 24S-OHC-induced SG formation and mitigated 24S-OHC-induced cell death. | Cell Death Discovery

Fig. 4: Inhibition of PERK axis suppressed 24S-OHC-induced SG formation and mitigated 24S-OHC-induced cell death.

From: Integrated stress response is involved in the 24(S)-hydroxycholesterol-induced unconventional cell death mechanism

Fig. 4

a–c Cells were treated with 50 μM 24S-OHC or 1 μM Thapsi for 3 h in presence or absence of 0.5 μM GSK2606414 (GSK). a Cells were then incubated with 10 μg/ml puromycin for final 15 min. Whole-cell lysates were subjected to immunoblotting using appropriate antibodies, as indicated. b, c Cells were subjected to immunofluorescence staining for TIA1 and G3BP1. b Representative confocal images. c Percentage of cells containing G3BP1-positive SGs. **P < 0.01. d Cells were treated with 30 μM 24S-OHC for 24 h in presence or absence of 0.5 μM GSK. Cell viability was measured by WST-8 assay.

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