Fig. 5: Effect of VDAC inhibitor on ferroptosis.

A After treatment of SK-Hep1 ρ⁺ cells with 5 μM erastin or 0.1 μM RSL3 in the presence or absence of 10 μM VBIT-4, cell death was evaluated by LDH release assay as in (Fig. 1E). B–D After treatment of SK-Hep1 ρ⁺ cells with 5 μM erastin in the presence or absence of 10 μM VBIT-4 as in A, accumulation of MitoSOX B, BODIPY-C11 C, and MitoPerOx D was evaluated as in (Figs. 3A, 2A, and 3D) (right). Representative histograms are shown (left). E–G After treatment of SK-Hep1 ρ⁺ cells with 0.1 μM RSL3 in the presence or absence of 10 μM VBIT-4 as in A, accumulation of MitoSOX (E), BODIPY-C11 (F), and MitoPerOx (G) was evaluated as in (Figs. 3A, 2A, and 3D) (right). Representative histograms are shown (left). Data represent means ± SD from three independent experiments. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test (A–G). (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant).