Fig. 6: Resistance to cell death and reduced accumulation of cellular ROS, lipid peroxides, or mitochondrial ROS by adenoviral expression of GPx4.
From: Mitochondrial event as an ultimate step in ferroptosis
A After infection of SK-Hep1 ρ+ cells with adenovirus expressing Ad-cytoGPx4 or Ad-mitoGPx4, cells were treated with 5 μM erastin or 0.1 μM RSL3 for 24 h. Cell death was measured by LDH release assay. B, C After treatment of SK-Hep1 ρ+ cells infected with adenovirus expressing Ad-cytoGPx4 or Ad-mitoGPx4) with 5 μM erastin (B) or 0.1 μM RSL3 (C), accumulation of cellular ROS, peroxidized lipids and mitochondrial ROS was determined by flow cytometry after staining with CM-H2DCFDA, BODIPY-C11 and MitoSOX, respectively (right lower). Representative histograms are shown (upper and left lower panels). Data represent means ± SD from three independent experiments. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant).
