Fig. 2: NUPR1 promotes HCC cell proliferation in vitro and induces tumor growth in vivo. | Cell Death Discovery

Fig. 2: NUPR1 promotes HCC cell proliferation in vitro and induces tumor growth in vivo.

From: The interplay of transcriptional coregulator NUPR1 with SREBP1 promotes hepatocellular carcinoma progression via upregulation of lipogenesis

Fig. 2

A, B Efficiency of overexpression and knockdown against human NUPR1 was verified by qRT-PCR assay (a) and western blot (b) and β-actin as an internal control. C The impact of aberrant NUPR1 expression on cell proliferation among HCC cells was detected by CCK8 assay. D The proliferation ability of MHCC-97H and SK-Hep1 cells overexpressed NUPR1 was detected by EdU assays, scale bars represent 20 µm. E Effects of NUPR1 on HCC xenograft tumor growth in vivo. Left: Representative images of tumors in nude mice after subcutaneous injection of MHCC-97H cells with LV-NUPR1 and LV-NC (n = 4). Middle: Column scatter plot of the xenograft tumor weight. Right: Tumor growth curve was plotted using xenograft tumor volume data. F NUPR1 expression in MHCC-97H xenograft tumor was detected by immunohistochemistry (left panels, scale bars 100 µm and right panels, scale bars 20 µm). G The images and statistical analyses display the colony formation of Huh7 and SMMC-7721 transfected with sh-NUPR1. H Xenograft growth in nude mice injected with SMMC-7721 and SMMC-7721-shNUPR1 (n = 5). Tumor photographs (left), tumor weight (middle) and tumor growth curve (right).

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