Fig. 5: NUPR1 affected key enzyme expression of de novo lipogenesis and lipid droplet contents. | Cell Death Discovery

Fig. 5: NUPR1 affected key enzyme expression of de novo lipogenesis and lipid droplet contents.

From: The interplay of transcriptional coregulator NUPR1 with SREBP1 promotes hepatocellular carcinoma progression via upregulation of lipogenesis

Fig. 5

A, C mRNA levels of critical lipogenesis enzymes in HCC cells with transient was detected by RT-qPCR. B, D Protein expression of critical lipogenesis enzymes in HCC cells with transient NUPR1 overexpression (b) or knockdown (d) was detected by Western blot. E The correlation between NUPR1 and SREBP1 was detected by immunohistochemistry in HCC patients (Pearson R = 0.4506, n = 25). F The correlation between NUPR1 and FASN was detected by immunohistochemistry (Pearson R = 0.5047, n = 38). G MHCC-97H and SK-Hep1 cells transfected with LV-NC or LV-NUPR1 stained with Bodipy 493/503 and images were acquired by confocal microscope.

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