Fig. 3: PC reduces expression of iodine metabolism-related genes and iodine uptake and promotes MAPK pathway signaling in PTC cells. | Cell Death Discovery

Fig. 3: PC reduces expression of iodine metabolism-related genes and iodine uptake and promotes MAPK pathway signaling in PTC cells.

From: Pyruvate carboxylase promotes malignant transformation of papillary thyroid carcinoma and reduces iodine uptake

Fig. 3

A Protein and B mRNA expression levels of TSHR, NIS, TPO, TG, and MAPK pathway signals in TPC-1 and KTC-1 cells, detected by western blot and RT-PCR, respectively. Protein expression quantified by ImageJ analysis of western blots. Full blots provided in the Supplementary File. C Quantification of NIS fluorescence. D Immunofluorescence detection of localization and expression of NIS in TPC-1 and KTC-1 cells (magnification ×20 objective); E Iodine uptake detected by 125I uptake assay in TPC-1 and KTC-1 cells. ***p < 0.001, **p < 0.01, *p < 0.05. Quantitative results based on three independent experiments.

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