Fig. 4: Reduced MAPK pathway signaling restores expression of iodine metabolism-related genes and iodine uptake in PC-overexpressed PTC cells. | Cell Death Discovery

Fig. 4: Reduced MAPK pathway signaling restores expression of iodine metabolism-related genes and iodine uptake in PC-overexpressed PTC cells.

From: Pyruvate carboxylase promotes malignant transformation of papillary thyroid carcinoma and reduces iodine uptake

Fig. 4

A mRNA expression of TSHR, NIS, TPO, and TG detected by RT-PCR and B protein expression of TSHR, NIS, TPO, TG, ERK1/2, and pERK1/2 detected by western blot in PC-overexpressed TPC-1 and KTC-1 cells with siRNAs or 5 µM SCH772984 knockdown of ERK1/2 signaling. Quantitative analysis of protein expression provided in Figure S6. Full blots provided in the Supplementary File. C Immunofluorescence detection and localization of NIS in PC-overexpressed TPC-1 and KTC-1 cells with siRNAs or 5 µM SCH772984 knockdown of ERK1/2 signaling (magnification 20× objective). Quantification of NIS fluorescence shown below. D Iodine uptake detected by 125I uptake assay in PC-overexpressed TPC-1 and KTC-1 cells with siRNAs or 5 µM SCH772984 knockdown of ERK1/2 signaling. ***p < 0.001, **p < 0.01, *p < 0.05. Results based on three independent experiments. Quantitative results represent three independent experiments.

Back to article page