Fig. 7: ZY-444 restores expression of iodine metabolism-related genes and iodine uptake in PTC cells. | Cell Death Discovery

Fig. 7: ZY-444 restores expression of iodine metabolism-related genes and iodine uptake in PTC cells.

From: Pyruvate carboxylase promotes malignant transformation of papillary thyroid carcinoma and reduces iodine uptake

Fig. 7

A mRNA expression of TSHR, NIS, TPO, and TG detected by RT-PCR and B protein expression of TSHR, NIS, TPO, TG, ERK1/2, and P-ERK1/2 detected by western blot in TPC-1 and KTC-1 cells with ZY-444 treatment. Full blots provided in the Supplementary File. C Quantitative analysis of protein expression. D Immunofluorescence detection and localization of NIS in TPC-1 and KTC-1 cells with ZY-444 treatment (magnification ×20 objective). Quantification of NIS fluorescence shown below. E Iodine uptake detected by 125I uptake assay in TPC-1 and KTC-1 cells with ZY-444 treatment. ***p < 0.001, **p < 0.01, *p < 0.05. Results expressed as mean ± SD of three independent experiments and shown in histograms on the right.

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