Fig. 2: CAFs promote the cancer stemness of RCC cells through secreting exosomes. | Cell Death Discovery

Fig. 2: CAFs promote the cancer stemness of RCC cells through secreting exosomes.

From: Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p

Fig. 2

A Immunofluorescence staining of α-SMA, Vimentin, and E-cadherin in CAFs and NFs, scale bar = 50 µm. B Western Blot analysis of α-SMA, Vimentin, and E-cadherin protein levels in CAFs, NFs, and ACHN cell line. C Representative images (Left) and histogram statistics (Right) from colony formation assay of RCC cells co-cultured with NFs, CAFs, or GW4968 pre-treated CAFs, scale bar = 0.5 cm. D Representative images (Left) and histogram statistics (Right) from sphere formation assay of RCC cells co-cultured with NFs, CAFs, or GW4968 pre-treated CAFs. E Representative images (Left) and histogram statistics (Right) from EdU assay of RCC cells co-cultured with NFs, CAFs, or GW4968 pre-treated CAFs, scale bar = 50 µm. F RT-qPCR analysis of cancer stemness relative genes OCT4 and ALDH1A1 expression in RCC cells co-cultured with NFs, CAFs, or GW4968 pre-treated CAFs, normalized to β-actin. G Western blot of OCT4 and ALDH1A1 expression in RCC cells co-cultured with NFs, CAFs, or GW4968 pre-treated CAFs, normalized to α-Tubulin. Cell experiment was repeated three times independently, P value between the two groups was obtained by Unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001.

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