Fig. 3: CAF-derived exosomes promote the cancer stemness of RCC cells. | Cell Death Discovery

Fig. 3: CAF-derived exosomes promote the cancer stemness of RCC cells.

From: Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p

Fig. 3

A Representative images of transmission electron micrograph of NFs-secreted exosomes (NF-Exo) and CAFs-secreted exosomes (CAF-Exo), scale bar = 100 nm. B Western blot analysis of exosome markers CD63, CD81, and TSG101 in NF-Exo and CAF-Exo. C Transfer of exosomes from CAFs to RCC cells examined by confocal microscope, scale bar = 50 µm. D Representative images (Left) and histogram statistics (Right) from sphere formation assay of NF-Exo or CAF-Exo treated RCC cells, scale bar = 100 µm. E Representative images (Left) and histogram statistics (Right) from colony formation assay of NF-Exo or CAF-Exo treated RCC cells, scale bar = 0.5 cm. F Representative images (Left) and histogram statistics (Right) from EdU assay of NF-Exo or CAF-Exo treated RCC cells, scale bar = 50 µm. G Western Blot analysis of OCT4 and ALDH1A1 expression in NF-Exo or CAF-Exo treated RCC cells, normalized to α-Tubulin. H RT-qPCR analysis of OCT4 and ALDH1A1 expression in NF-Exo or CAF-Exo treated RCC cells, normalized to β-actin. Cell experiment was repeated three times independently, P value between two groups was obtained by Unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001.

Back to article page