Fig. 6: CAF-secreted exosomal miR-181d-5p enhances the cancer stemness of RCC cells. | Cell Death Discovery

Fig. 6: CAF-secreted exosomal miR-181d-5p enhances the cancer stemness of RCC cells.

From: Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p

Fig. 6

A RT-qPCR analysis of miR-181d-5p expression in RCC cells treated with NF-Exo, CAF-Exo, or CAF-181sponge-Exo, normalized to U6; B Representative images (Left) and histogram statistics (Right) of colony formation in RCC cells treated with NF-Exo, CAF-Exo, or CAF-181sponge-Exo, scale bar = 0.5 cm; C Representative images (Left) and histogram statistics (Right) of sphere formation in RCC cells treated with NF-Exo, CAF-Exo, or CAF-181sponge-Exo, scale bar = 100 µm; D Representative images (Left) and histogram statistics (Right) of EdU assay in RCC cells treated with NF-Exo, CAF-Exo, or CAF-181sponge-Exo, scale bar = 50 µm; E RT-qPCR analysis of mRNA levels in RCC cells treated with NF-Exo, CAF-Exo, or CAF-181sponge-Exo, normalized to β-actin; F Western Blot analysis of protein levels in RCC cells treated with NF-Exo, CAF-Exo, or CAF-181sponge-Exo, normalized to α-Tubulin. Cell experiment was repeated three times independently, P value between two groups was obtained by Unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001.

Back to article page