Fig. 2: Recombinant NOP53-mEGFP undergoes LLPS in vitro.
From: NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance
A Coomassie staining of purified NOP53-mEGFP protein. B NOP53-mEGFP protein formed droplets at different temperature. Two micromolar protein was used. C NOP53-mEGFP droplets that formed in buffers containing 150 mM NaCl and 25 mM Tris-HCl (pH 7.4) were observed with confocal microscopy. D The impact of protein concentration, NaCl concentration and pH on the formation of NOP53-mEGFP droplets. The fluorescence intensity of droplets is presented as the mean intensity × area. E NOP53-mEGFP droplets were disrupted by dilution and increasing NaCl concentrations. Droplets formed in buffer containing 2 μM NOP53-mEGFP and 150 mM NaCl at pH 7.4; high salt, 500 mM NaCl. F Two in vitro-formed NOP53-mEGFP droplets fused to form a larger droplet.
