Fig. 3: IDR1 drives the LLPS of NOP53.
From: NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance
A The disordered region of NOP53 was analyzed with PONDR (www.pondr.com). B IDR1-Cry2-mCherry was expressed in cells, which were stimulated with blue light to induce condensation. C IDR1-Cry2-mCherry droplets fused to form a larger droplet upon stimulation with blue light. D FRAP of IDR1-GFP puncta in HEK293T cells. E Coomassie staining of purified NOP53-IDR1-GFP protein. F NOP53-IDR1-GFP protein formed droplets at different temperatures. Ten micromolar protein was used. G PEG-8000 enhanced the formation of NOP53-IDR1-GFP droplets. Two micromolar protein was used. H The impact of protein concentration and pH on the formation of NOP53-IDR1-GFP droplets. The fluorescence intensity of droplets is presented as the mean intensity × area. I, J FRAP of IDR1-GFP droplets in vitro. K Two in vitro-formed IDR1-GFP droplets fused to form a larger droplet. L IDR1-GFP droplets were disrupted by dilution and increasing NaCl concentrations. Droplets formed in buffer containing 10 μM IDR1-GFP and 150 mM NaCl at pH 7.4; high salt, 500 mM NaCl. For (D and J), n = 3 punctum analyzed in 3 independent experiments. Data are mean ± SD.
