Fig. 5: NOP53 promotes tumor radio-resistance.
From: NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance
A CCK-8 assay showed that NOP53 silencing abolished the growth of HCT-8 cells. B Colony formation assay showed that NOP53 enhanced the radio-resistance of HCT-8 cells. C, D NOP53 silencing increased p53 and p21 protein (C) and mRNA (D) level in HCT8 cells treating with/without irradiation. HCT-8 cells were transfected with NOP53 siRNA for 48 h before irradiation (10 Gy X-rays), then cultured for 24 h before western blotting analysis. E NOP53 depletion-induced p21 upregulation was dependent on p53. F NOP53 was localized to the nucleolus in CRC tissues. G, H CRC patients with higher NOP53 level were more resistant to radio-chemotherapy. TRG, Tumor regression grade. I, J. Higher NOP53 expression was correlated with a shorter progression-free survival and disease-free survival time of CRC patients. For (G–J), CRC tissues from 148 patients were analyzed. Data are expressed as mean ± SD; *,P < 0.05; **,P < 0.01; ***,P < 0.001; ns, no significance.
