Fig. 1: Effect of LXN deficiency on hematopoietic stem cells in bone marrow and immune cells in peripheral blood.

A Volcano plot of gene expression changes of bone marrow from WT and LXN KO mice. B, C Heatmap of RNA-seq data showing the up- (B) and downregulation (C) genes in LXN deficiency bone marrow. D GSEA of RNA-seq data from the bone marrow of LXN KO versus WT mice using the signaling pathways regulating stem cell pluripotent, pathways in antigen processing and presentation gene set annotated in the KEGG. NES, normalized enrichment score. E Representative FACS plots and frequencies of Lin⁻Sca1⁺ckit⁺ in bone marrow from WT and LXN KO mice. F, G Representative FACS plots and frequencies of CD45⁺ (F), F4/80⁺CD11b⁺ (G) cells in peripheral blood from WT and KO mice. H Representative FACS plots and frequencies of B220⁺ and CD3⁺ cells in peripheral blood, spleen and bone marrow from WT and LXN KO mice. n = 6, *P < 0.05, **P < 0.01, n.s. no significance.