Fig. 3: LXN-deficient macrophages inhibit T cell activity in vitro. | Cell Death Discovery

Fig. 3: LXN-deficient macrophages inhibit T cell activity in vitro.

From: Loss of LXN promotes macrophage M2 polarization and PD-L2 expression contributing cancer immune-escape in mice

Fig. 3

A Flow cytometry analysis of T cells isolated from spleen of WT and LXN KO mice. Representative FACS plots (left) and frequencies (right) of T cells. n = 4, n.s. no significance. B T cells from WT and LXN KO mice were activated by CD3 (5 μg/mL) antibodies for 48 h. The activity of T cells was evaluated by measuring the expression of CD44, IFN-γ and IL-2, by qRT-PCR. n = 3, **P < 0.01, n.s. no significance. C–E Isolated T cells (1 ×;104 per well) were co-cultured in 96-well plates with a 2:1 ratio of the WT or LXN KO macrophage for 72 h. T cells were analyzed by flow cytometer (C), the count of CD3+, CD3+CD4+ and CD3+CD8+ cells are showed (D). The production of CD44, INF-ɤ and IL-2 were determined by qRT-PCR (E). n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significance. Data are representative of three independent experiments.

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