Fig. 6: LXN deficiency increases enhances CD45⁺CD11b⁺PD-L2⁺ macrophages in AOM/DSS-induced colonic tumor tissues.

A Gross view of colonic tumor of AOM/DSS-treated WT and LXN KO mice. Tumor number and size were counted (n = 6 mice). **P < 0.01. B Flow cytometry analysis of B cell (CD3⁻B220⁺), T cells (CD3⁺B220⁻) and the different subsets (CD8⁺T cells and CD4⁺T cells) in colon tissue in WT and LXN KO mice treated with AOM/DSS. C, D Flow cytometry analysis of macrophages (F4/80⁺CD11b⁺), the different subsets (M1, CD16/32⁺CD206⁻; M2^, CD16/32⁻CD206⁺) (C) and CD45⁺CD11b⁺PD-L2⁺ cells (D) in colon tissue in WT and LXN KO mice treated with AOM/DSS. n = 6, *P < 0.05, **P < 0.01. Data are representative of three independent experiments.