Fig. 2: SAO-1 and DLC-1 promote C. elegans germ cell apoptosis. | Cell Death Discovery

Fig. 2: SAO-1 and DLC-1 promote C. elegans germ cell apoptosis.

From: Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline

Fig. 2

A Schematic representation of the adult C. elegans hermaphrodite germline. B Representative confocal images of the apoptotic corpses labeled by CED-1::GFP (black asterisk) in control, sao-1 (RNAi), sao-1(ΔFL), sao-1(ΔC), Ex[sao-1::rfp];sao-1(ΔFL) and Ex[sao-1::rfp];sao-1(ΔC). Worms were imaged at 48 h after L4 molt. Asterisks: apoptotic corpses. The schematic representation of sao-1(ΔC) please refer to Fig. 4C. C Quantification of the number of apoptotic corpses in B, each dot in the chart represents a single worm. D Representative confocal images of the apoptotic corpses in ced-9(n1653) in control, sao-1(RNAi), and dlc-1(RNAi). E Quantification of the number of apoptotic corpses in D, each dot in the chart represents a single worm. F Representative confocal images of CED-4::GFP in control, sao-1(RNAi), sao-1(ΔFL), dlc-1(RNAi), ced-9(RNAi), sao-1(RNAi);ced-9(RNAi), sao-1(ΔFL);ced-9 (RNAi), and dlc-1(RNAi);ced-9(RNAi). G Quantification of CED-4::GFP intensity in F, each dot in the chart represents a single nuclear. Data were analyzed by two-tailed Student’s t test; Error bars, ±SEM; **p < 0.01, ***p < 0.001. a.u., arbitrary unit. Scale bar, 50 μm.

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