Fig. 3: METTL1 is demanded for tumor proliferation and metastasis of BCa in vivo. | Cell Death Discovery

Fig. 3: METTL1 is demanded for tumor proliferation and metastasis of BCa in vivo.

From: METTL1 drives tumor progression of bladder cancer via degrading ATF3 mRNA in an m7G-modified miR-760-dependent manner

Fig. 3

A Western blot assay showed METTL1 was deleted by shMETTL1. GAPDH was used for the normalization control. B–G Subcutaneous xenograft tumor model (BALB/c nude mice). B The tumor growth curve of subcutaneous xenografts plotted by the tumor size (width2 × length × 0.52) measured with vernier caliper every 3 days (n = 10 each group). C–E Subcutaneous xenografts in METTL1 depletion group grew too slow to be figured out by the naked eyes at end point of observation. F–J Fluorescence intensity measured by IVIS system was weaker in shMETTL1 group than shNC group. H–K Experimental metastases mice model (BALB/c nude mice) by caudal vein injection. H, I Fluorescence intensity of and the amount of metastasis sites measured by IVIS system decreased in shMETTL1 group. J Lymph node metastases and osseous metastases obtained from shNC group. K H&E staining of lymph node metastases. The red arrows point to abnormal cells with pathologic mitosis. Statistical significance was determined by Student’s t test: ***P < 0.001.

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