Fig. 7: Representative images from proximity ligation assay (PLA): Assays were performed on Strip2−-ESCs vs Strip2+-ESCs at different time point of differentiation (0-day, 1-day and 4-day EBs).
From: Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells
A Absence of proximity (distance < 40 nm) red dots between Strip2 and ZFP57 in Strip2−-ESCs condition in all three time points. B Evidence of proximity (distance < 40 nm) red dots found between Strip2 and ZFP57 in all three time points. C Absence of proximity (distance < 40 nm) red dots between Strip2 and TRIM28 in Strip2−-ESCs condition in all three time points. D Strip2 and TRIM28 proximity (distance < 40 nm) red dots were found only in 0-day ESCs and 1-day EBs but not in 4-day EBs. Nuclei are counterstained with DAPI (blue fluorescent stain). Scale bar: 50 μm.
