Fig. 3: Impact of short-term stimulation of ASCs with adipogenic differentiation medium on ARNTL1 and ARNTL2 expression. | Cell Death Discovery

Fig. 3: Impact of short-term stimulation of ASCs with adipogenic differentiation medium on ARNTL1 and ARNTL2 expression.

From: The circadian transcription factor ARNTL2 is regulated by weight-loss interventions in human white adipose tissue and inhibits adipogenesis

Fig. 3

A ARNTL2 mRNA expression. Values are given as mean ± SEM of n = 3 individual donors. Statisitcal analysis between groups was achieved using One-way ANOVA with Dunnett´s multiple comparison test. B A representative ARNTL2 Western blot of n = 3 different donors is shown. β-Actin served as loading control. C Densitometric quantification of ARNTL2 Western blots of n = 3 individual donors. Values are shown as mean ± SEM. Statistical comparison was done with the paired t test. D ARNTL1 mRNA expression. Values are given as mean ± SEM of n = 3 individual donors. Statisitcal analysis between groups was achieved using One-way ANOVA with Dunnett´s multiple comparison test. E A representative ARNTL1 Western blot of n = 3 different donors is shown. β-Actin served as loading control. F Densitometric quantification of ARNTL1 Western blots of n = 3 individual donors. Values are shown as mean ± SEM. Note: scaling of y-axis is different in (C) and (F). DM differentiation medium.

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