Fig. 6: Enzalutamide binds to AR in 22RV1 cells, influencing the interaction between AR and ARv7. | Cell Death Discovery

Fig. 6: Enzalutamide binds to AR in 22RV1 cells, influencing the interaction between AR and ARv7.

From: Tautomycin and enzalutamide combination yields synergistic effects on castration-resistant prostate cancer

Fig. 6

A ITDRFCETSA experiments performed for DHT in the presence of 50 μM enzalutamide in 22RV1 cells for 1 h. B CETSA experiments were performed following indicated heat shocks in 22RV1 cells after treating with 10 μM enzalutamide for 48 h. C The protein levels of ARv7 were analyzed using Western blot in 22RV1 cells transfected with FL-AR siRNA and treated with indicated concentrations of tautomycin and enzalutamide for 48 h. D The ubiquitin level of ARv7 was evaluated using co-immunoprecipitation in 22RV1 cells in the presence of 10 μM MG132 for 48 h with or without knockdown of full-length AR. E A schematic model for the mechanism through which a combination of tautomycin and enzalutamide inhibits the proliferation of prostate cancer. P < 0.05 was accepted as significant, ns as not significant.

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