Fig. 5: Interaction of PEVs with Jurkat cells and analysis of cell invasion. | Cell Death Discovery

Fig. 5: Interaction of PEVs with Jurkat cells and analysis of cell invasion.

From: Proteomic and functional profiling of platelet-derived extracellular vesicles released under physiological or tumor-associated conditions

Fig. 5

A Representative images of CFSE-loaded PEVs interacting with Jurkat cells. For Jurkat cells treated with PEVs-THR or PEVs-BCC and untreated cells (NT), three representative examples including green-fluorescence images of Channel 2 (Ch2), brightfield images of Channel 4 (Ch4) and Merge of Ch2 and Ch4 are shown. B Analysis of PEVs-Jurkat cells interaction by flow cytometry. Jurkat cells were co-cultured for 24 h with PEVs obtained from CFSE-labeled platelets upon stimulation with thrombin or MDA-MB-231 cells. The green-fluorescent signal of PEVs associated to Jurkat cells was quantified as Median Fluorescence Intensity (MFI). The plot reports the mean ± SD of three independent experiments. ***p < 0.0001 (sample vs NT). C Jurkat cells migration evaluated by transwell assay. Cells that moved in the bottom chamber were collected and counted using a hemocytometer. The data are expressed as number of migrated cells and the graph shown the mean ± SD of four independent experiments with *p < 0.05.

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