Fig. 5: The underlying mechanism of autophagy regulation in ADSC. | Cell Death Discovery

Fig. 5: The underlying mechanism of autophagy regulation in ADSC.

From: Protection of adipose-derived mesenchymal stromal cells during acute lung injury requires autophagy maintained by mTOR

Fig. 5

ADSCs were cultured in vitro and treated with a type III PI3K inhibitor 3-mA or mTOR inhibitor (rapamycin). MLE-12 cells were cocultured with treated ADSCs via a Transwell system and stimulated with LPS. A The level of inflammatory cytokines IL-2, TNF-α, and IFN-γ in the supernatants of MLE-12 cells cultured with different groups of ADSCs were detected by ELISA. ***P < 0.001. B Autophagy in the different groups of ADSCs was detected using an mRFP-GFP-LC3 dual-fluorescence autophagy indicator system under a confocal microscope. ***P < 0.001; ###P < 0.001; $$$P < 0.001. C The levels of autophagy-related protein indicators were detected by WB. ***P < 0.001. D Autophagosomes were detected by electron microscopy. Red arrows indicate the autophagosomes. The experiment was repeated triple. Data are shown as means ± SD, *P < 0.05, **P < 0.01. (determined by unpaired t test or one-way ANOVA with Tukey comparisons.).

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