Fig. 3: Knockdown of CHES1 impaired the proliferation and invasion of TNBC in vitro and in vivo. | Cell Death Discovery

Fig. 3: Knockdown of CHES1 impaired the proliferation and invasion of TNBC in vitro and in vivo.

From: Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer

Fig. 3

A CCK8 assay evaluated the effect of shCHES1 on the proliferation of MDA-MB-231 and BT549 cells. ***p < 0.001. B, C Scratch wound-healing assay showed the effect of shCHES1 on the movement of MDA-MB-231 (left) and BT549 (right) cells. **p < 0.01, ***p < 0.001. D Transwell assay determined the effect of shCHES1 on the migration and invasion of MDA-MB-231 and BT549 cells. E Colume diagram shown the statistical significance between shCtrl and shCHES1 on the metastasis potential of TNBC. Data were shown means ± SD, **p < 0.01, ***p < 0.001. F, G Stably knockdown of CHES1 exhibited a significant reduced role in tumor volume (F) and tumor weight (G) compared to control group (each group n = 5). Data of tumor volume were shown with means ± SD, tumor weights were shown with means ± SD, **p < 0.01. H The captured image of tumors showed the tumor size of two groups. I IHC assays showed the indicated proteins expression in the tumor tissues from two groups. J Qualified bioluminescence imaging showed the lung metastasis of shCtrl or shCHES1 groups in tail-vain metastasis model. K Histogram showed the average radiance of metastases in two groups. Data were shown the average radiance ±SEM, *p＜0.05.

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