Fig. 3: Increased expression of Ripk3 in cells lacking primary cilia. | Cell Death Discovery

Fig. 3: Increased expression of Ripk3 in cells lacking primary cilia.

From: Primary cilia suppress Ripk3-mediated necroptosis

Fig. 3

A, B Quantitative real-time PCR of several cell death-related genes in mIMCD3 cells revealed upregulation of necroptosis players in non-ciliated cells: A Ckc versus Nckc (n = 3); B control versus Myo5a−/− (n = 3). Cells were treated with TNFα (TNF, 4 ng/100 µl) and cycloheximide (CHX, 2 µg/100 µl) for 16 h or with DMSO. Statistical analysis was performed by using a one-way ANOVA followed by a two-sided Student’s t test (p value: >0.001***; 0.002**; 0.033*; ns = 0.12). C Immunoblot analysis of lysates from untreated ciliated (Myo5+/+/Ckc) and non-ciliated (Myo5a−/−/Nckc) cells using Ripk3 (~57 kDa) and HSP27 (~27 kDa) antibodies. Pan-actin (~30 kDa) or beta-tubulin (~55 kDa) were used as controls.

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