Fig. 5: Genetic targeting of ciliogenesis leads to cell death and increased Ripk3 expression in vivo. | Cell Death Discovery

Fig. 5: Genetic targeting of ciliogenesis leads to cell death and increased Ripk3 expression in vivo.

From: Primary cilia suppress Ripk3-mediated necroptosis

Fig. 5

A PAS staining of kidneys from Kif3afl/fl:Ksp:cre+/− and Kif3fl/wt:Ksp:cre+/− mice at a postnatal age of 4 days (scale bar 200 µm) and 28 days (scale bar 500 µm) showing the loss of kidney architecture and cyst formation over time. TUNEL staining (scale bar 100 µm) indicates cell death. B Quantitative real-time PCR of several cell death genes showing upregulation of necroptosis-specific genes in mouse tissue lacking primary cilia (n = 3). Statistical analysis was performed by using a one-way ANOVA followed by a two-sided Student’s t test (p value: >0.001***; 0.002**; 0.033*; ns = 0.12). Control, heterozygous transgenic mice. C Immunoblot analysis of 28-day-old mice for Ripk3 expression (~57 kDa; n = 3 individual animals shown).

Back to article page