Fig. 6: Loss of the functional but not structural ciliary protein Nphp1 enhances the necroptotic response. | Cell Death Discovery

Fig. 6: Loss of the functional but not structural ciliary protein Nphp1 enhances the necroptotic response.

From: Primary cilia suppress Ripk3-mediated necroptosis

Fig. 6

A Immunoblot analysis demonstrating Nphp1 deficiency in Nphp1−/− cells and confirming re-expression of FLAG.Nphp1 by using Nphp1 (~83 kDa). B Neutral-red assay in Nckc proficient and deficient in Nphp1. RCD induction with TNFα (TNF, 4 ng/100 µl) and cycloheximide (CHX, 2 µg/100 µl) for 16 h. Additionally, caspase-8 inhibitor emricasan (Em, 10 µM) and necroptosis inhibitor necrostatin-1s (Nec1s, 40 µM) were used for 16 h (n = 3). Knockout of Nphp1 resulting in increased necroptotic death. C Immunoblot of phospho-Mlkl (~56 kDa) in Nphp1 proficient and deficient cells upon TC and TCE treatment for 8 h. Pan-actin was used as a loading control (n = 3).

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