Fig. 3: KDM5C inhibits trophoblast outgrowth in a villi explants model. | Cell Death Discovery

Fig. 3: KDM5C inhibits trophoblast outgrowth in a villi explants model.

From: Elevated histone demethylase KDM5C increases recurrent miscarriage risk by preventing trophoblast proliferation and invasion

Fig. 3

A The villi explants were seeded in Matrigel-coated plates, and western blotting assay was performed on KDM5C protein from explants treated with either lenti-control or lenti-KDM5C for 72 h. B Level of KDM5C protein was determined in HTR-8 cells transduced with control or KDM5C siRNAs using western blotting assay. C The villi explants were plated on Matrigel-coated plates, and western blotting assay was performed on KDM5C protein in extravillous explants transduced with either NC siRNA or KDM5C siRNA-2 for 72 h. D, E Villi explants from HCs were cultured on Matrigel-coated plates. Time course images of the villi explants treated with either lenti-control or lentiviral vector carrying the KDM5C gene (lenti-KDM5C) were taken using light microscopy after 24 and 72 h of culture ex vivo; Scale bar = 250 μM. Statistical assay of villous tip migration (%), n = 6 for each group. F, G Villi explants were cultured in Matrigel-coated plates. Time course images of the explants transfected with either control siRNA or KDM5C siRNA-2 were obtained using microscope after 24 and 72 h of culture ex vivo; Scale bar = 250 μM. Statistical assay of villous tip migration (%), n = 6 for each group. Data are shown as the mean ± SEM; Student’s t test was used to evaluate the statistical significance; **p < 0.01.

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