Fig. 4: KDM5C inhibits substantial regulators of trophoblast proliferation and invasion. | Cell Death Discovery

Fig. 4: KDM5C inhibits substantial regulators of trophoblast proliferation and invasion.

From: Elevated histone demethylase KDM5C increases recurrent miscarriage risk by preventing trophoblast proliferation and invasion

Fig. 4

A Heatmap indicating differentially expressed genes between HTR-8 cells stably transduced treated with either control vector or KDM5C overexpression vector, or between HTR-8 cells treated with either NC shRNA or KDM5C shRNA-2. GSEA of the expression profile of HTR-8 cells under KDM5C overexpression or knockdown conditions using a cell cycle process-associated signature (B, C), cell cycle phase-associated signature (D, E), cell proliferation-negative associated signature (F, G), and trophoblast cell migration-associated signature (H, I). J Heatmap indicating differential gene expression of HTR-8 cells under KDM5C overexpression (left panel) or knockdown (right panel) conditions, which focused on a set of pro-proliferation/invasion genes and anti-proliferation/invasion genes. qRT-PCR assay of the mRNA levels of genes in HTR-8 cells stably transduced with vector control, KDM5C-expressing vector (K), control shRNA or two KDM5C shRNAs (L); n = 3 for each group. K Student’s t test; L the ANOVA test. Data are shown as the mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.

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