Fig. 7: TGFβ2 and RAGE negatively correlate with KDM5C in RM patients. | Cell Death Discovery

Fig. 7: TGFβ2 and RAGE negatively correlate with KDM5C in RM patients.

From: Elevated histone demethylase KDM5C increases recurrent miscarriage risk by preventing trophoblast proliferation and invasion

Fig. 7

Immunolabeling of villi tissue from HCs and RM patients with anti-TGFβ2 antibody (A, B), or anti-RAGE antibody (C, D), which were visualized using a HRP kit. Sections were hematoxylin counterstained, and cell positivity was quantified using Image-Pro Plus 6.0 software (n = 10); Scale bar = 100 μM. STB, syncytiotrophoblast; CTB, cytotrophoblast; EVT: extravillous trophoblast. The mRNA levels of KDM5C (E), TGFβ2 (F), and RAGE (G) found in villi from HCs (n = 25) and RM patients (n = 29) were detected using qRT-PCR. (H, I) level of KDM5C mRNA was negatively associated with level of TGFβ2 or RAGE mRNA in RM patients’ villous tissues and HCs. Data are shown as the mean ± SEM; Student’s t test was used to evaluate the statistical significance; *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page