Fig. 1: The effects of BMP9 on TAZ expression in MSCs and MMCs, TAZ was a direct target of BMP9/Smad signaling. | Cell Death Discovery

Fig. 1: The effects of BMP9 on TAZ expression in MSCs and MMCs, TAZ was a direct target of BMP9/Smad signaling.

From: TAZ promotes osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12, and MEFs induced by BMP9

Fig. 1

A–D The protein expression level of TAZ was detected by Western blot after Ad-BMP9 or Ad-GFP infection for 24 h. E–J The nucleoprotein and cytoplasmic protein expression levels of TAZ were detected by western blot after Ad-BMP9 or Ad-GFP infection for 24 h. K Prediction of Smad1/5/8 binding sites in the TAZ promoter region. L Enrichment of Smad1/5/8 on the PP-1, PP-2, PP-3, and PP-4 fragment of the TAZ promoter were measured by ChIP in MSCs. The data were shown as mean ± SD for three separated experiments. (*P < 0.05, **P < 0.01, ***P < 0.001) MSCs mesenchymal stem cells, MMCs murine multi-lineage cells, ChIP chromatin immunoprecipitation, Ad-GFP adenovirus carrying green fluorescent protein gene, Ad-BMP9 adenovirus carrying bone morphogenetic protein 9 gene, IgG immunoglobulin G, GAPDH, glyceraldehyde 3-phosphate-dehydrogenase, TAZ transcriptional co-activator with PDZ-binding motif.

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