Fig. 1: CAFs confer growth and radioresistance of breast cancer cells. | Cell Death Discovery

Fig. 1: CAFs confer growth and radioresistance of breast cancer cells.

From: Cancer-associated fibroblasts induce growth and radioresistance of breast cancer cells through paracrine IL-6

Fig. 1

A Morphological features of primary fibroblasts isolated from fresh breast cancer tissue. Scale bars 50 μm. B αSMA and FAP expression in primary fibroblasts was detected using western blotting. All the primary fibroblasts expressed high levels of αSMA and FAP, presenting characteristics of CAFs. C Immunofluorescence of αSMA and FAP in primary fibroblasts. D IHC labeling of αSMA in breast cancer tissue. Scale bars, 100 μm. E, F Colony formation assay showing that breast cancer cells treated with CAF conditioned medium formed more colonies than the control cells. G Clonogenic survival assay showing that treatment with CAF conditioned medium increased the survival fraction of breast cancer cells. Results are shown as means ± SD, n = 3, *P < 0.05, **P < 0.01.

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