Fig. 3: CAFs secreted IL-6 to induce the growth and radioresistance of breast cancer cells. | Cell Death Discovery

Fig. 3: CAFs secreted IL-6 to induce the growth and radioresistance of breast cancer cells.

From: Cancer-associated fibroblasts induce growth and radioresistance of breast cancer cells through paracrine IL-6

Fig. 3

A Cytokine antibody array analysis of the growth factors secreted by CAFs. IL-6, FGF-9, MIF, TNFRSF11, and TIMP1 were secreted at high levels from CAFs. B The expression of IL-6 was detected via IHC in adjacent normal tissue and breast cancer tumor tissue. Scale bars, 100 μm. C Western blotting analysis the p-STAT3 and STAT3 levels in T47D, MCF-7, and MDA-MB-231 cells. The cells were grown in CAF conditioned media for 12 h. D The concentration of IL-6 in conditioned media from paired normal tissue-associated fibroblasts (NAFs) and CAFs were analyzed by ELISA. E IL-6 was used to treat MDA-MB-231, MCF-7, and T47D cells for 6. The levels of STAT3 and p-STAT3 were analyzed using western blot. F, G Colony formation assay showing that IL-6 treatment increased the breast cancer cell colony numbers. H IL-6 treatment increased the survival fractions of breast cancer cells according to a clonogenic survival assay. The results are displayed as means ± SD, n = 3, *P < 0.05, **P < 0.01.

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