Fig. 4: High-concentration PGD2 induced ROS-mediated DNA damage and enhanced drug cytotoxicity in DLBCL cells. | Cell Death Discovery

Fig. 4: High-concentration PGD2 induced ROS-mediated DNA damage and enhanced drug cytotoxicity in DLBCL cells.

From: PGD2 displays distinct effects in diffuse large B-cell lymphoma depending on different concentrations

Fig. 4

A High-concentration PGD2 increased the mean fluorescence intensity of DLBCL cells loaded with DCFH-DA probe, indicating the intracellular accumulation of ROS. B High-concentration PGD2 promoted the phosphorylation of key regulators in DNA damage pathway. C Immunofluorescent images indicated that high-concentration PGD2 increased the expression of p-H2AX in DLBCL cells. Bar = 20 μm. D Representative images and quantification of the tail in comet assay. Bar = 20 μm. E High-concentration PGD2 enhanced the cytotoxicity of Adriamycin (ADR) and Bendamustine (BEN) in LY1 and LY3 cells. F High-concentration PGD2 increased the expression of Bcl-2 in DLBCL cells. G, H High-concentration PGD2 was observed to enhance the cytotoxicity of venetoclax in DLBCL cells. Cells were treated with drugs for 48 h. Data are shown as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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