Fig. 6: Effects of AST on the proliferation and apoptosis of Aβ25-35-damaged HT22 cells.
From: Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice
A Cell viability was detected in HT22 cells with different concentrations of Aβ25-35 (0–160 μM Aβ25-35, 24 h) by CCK8 assay. B Cell viability was detected in Aβ25-35-injured HT22 cells (20 μM Aβ25-35, 24 h) with different concentrations of AST (4 h before Aβ25-35 treatment) by CCK8 assay. C Cell viability was detected in HT22 cells with different concentrations of AST (0–160 μM AST, 24 h) by CCK8 assay. Data were presented as mean ± SD, *p < 0.05 vs 0 μM. D Effect of AST on the morphological alteration of HT22 cells was observed by immunofluorescent staining. E, F Flow cytometry analysis indicated the anti-apoptotic effect of AST on the apoptosis of HT22 cells. Data were presented as mean ± SD, n = 3/group. ***p < 0.001 vs control group, ##p < 0.01 vs AD group, @p < 0.05 vs AST + AD group.
