Fig. 7: The expressions of LC3BII/LC3BI, p62, Beclin-1, and LAMP-1 in HT22 cells were detected by Immunofluorescent staining and WB detection.
From: Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice
A Observation of LC3B, p62, Beclin-1, and LAMP-1 expression in HT22 cells by immunofluorescent staining, Scale bar = 100 μm. B Representative bands of LC3BII/LC3BI, p62, Beclin-1, and LAMP-1 in HT22 cells by WB detection. C–F Statistical analysis of the corresponding LC3BII/LC3BI, p62, Beclin-1, and LAMP-1 protein bands. Data were presented as mean ± SD, n = 3/group. **p < 0.01 vs control group, ##p < 0.01 and ###p < 0.001 vs AD group, @@p < 0.01 and @@@p < 0.001 vs. AST + AD group. G Representative bands of LC3BII/LC3BI, p62, Beclin-1, and LAMP-1 were detected by WB after the administration of Baf A1 in AD cell model. H–K Statistical analysis of the corresponding LC3BII/LC3BI, p62, Beclin-1, and LAMP-1 protein bands. Data were presented as mean ± SD, n = 3/group. **p < 0.01 vs AST + AD group.
