Fig. 2: Assessment of hypoxia and cell viability in PDAC organoids cultures and PDAC organoids-PSCs co-cultures.
From: A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment
Cells were grown for 4 days in the OrganoPlate® 2-Lane until used for subsequent analyses. A shows representative images of a hypoxia marker staining, images were acquired by confocal microscopy. B Hypoxia probe fluorescence intensity was quantified and data normalized to the probe in normoxic conditions. Data is shown as fold change of normoxic conditions (N = 3, n = 3). C Live and Dead assay staining with Hoechst, DraQ7 and Calcein-AM was used to access viability of the cultures. D shows the percentage of dead cells compared to the total cell number in cultures (N = 3, n = 3). The data was compared with Ordinary one-way ANOVA and Tukey’s multiple comparison test and shown are mean and SD (****p ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05). Confocal images were acquired on the ImageXpress Micro Confocal (Molecular Devices, US).
