Fig. 3: Hypoxia influences the transcriptional state of PDAC organoids (co-)cultures.
From: A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment
Cells were grown under 1% O2 or 20% O2, for 4 days until harvested for RNA isolation, cDNA synthesis and qPCR. A Gene expression analysis of cells grown in hypoxia vs. normoxia. Fold expression changes in normoxia and hypoxia in monoculture and co-culture. The values were normalized to β-actin expression and to normoxic culture conditions to determine the expression differences in hypoxia. (N = 3, 24 chips were pooled for each sample) B RNA Sequencing data of PDAC organoids grown in normoxia and hypoxia. The data is depicted with the Hallmark database and shows pathways upregulated in normoxia (above 0) and pathways upregulated in hypoxia (below 0). The color indicates the -log10(p.adjusted) (N = 1, 48 chips were pooled for each sample). C RNA-Sequencing data of PDAC organoids in co-culture with PSCs grown in normoxia and hypoxia. The data is depicted with the Hallmark database and shows pathways upregulated in normoxia (above 0) and pathways upregulated in hypoxia (below 0). The color indicates the -log10 (p.adjusted). (N = 1, 48 chips were pooled for each sample). Pathways with P < 0.05 are depicted. The graph with all up-, and downregulated genes are depicted in Fig. S2.
