Fig. 4: Chemotherapy treatment effect under different oxygen tension.
From: A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment
PDAC organoids were grown in monoculture and co-culture with PSCs under normoxia and hypoxic conditions. The cells were grown for 4 days on the OrganoPlate® and subjected to several combinations of (chemo)therapeutics for 72 h, after which their survival was analyzed with Cell Titer Glo 3D viability assay. The graphs show the survival of the cultures normalized to a no-treatment control (medium only). Cells were subject to 1 µM Gemcitabine (G) alone and in combination with other compounds (shown in graphs A and B). Shown are mean + − SD and the data points represent individual chips (N = 3, n = 3). Statistical analysis shows results of a 2-way ANOVA with Tukey’s multiple comparisons (****p ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05).
