Fig. 2: GSDMD deficiency mitigates brain damage caused by MCAO.
From: Gasdermin D inhibition ameliorates neutrophil mediated brain damage in acute ischemic stroke
A Western blot analysis of GSDMD protein expression in the brain tissue of GSDMD−/− mice compared to WT mice (n = 3). B Kaplan–Meier survival curves comparing post-MCAO survival of WT mice (n = 35) to that of GSDMD−/− mice (n = 50). GSDMD deficiency improved neurological functions, as indicated by the mNSS (n = 15) (C) and cylinder test (n = 13) (D). E Representative magnetic resonance imaging and quantitative analysis of the infarcted brain on 3 days after stroke in GSDMD−/− mice compared with WT mice (n = 5); the infarct area is shown in white. F Representative Nissl staining images of the cortex in each group. Scale bars, 50 μm. G Representative histology images showing TUNEL staining (n = 4). Scale bars, 50 μm. The bar graph shows the percentages of TUNEL-positive cells. The data are presented as the mean ± SD. Statistical analysis: two-tailed unpaired Student’s t test (A, E and G), log-rank (Mantel–Cox) test (B) or multiple two-tailed unpaired Student’s t test (C and D). ns not significant, &P < 0.05, &&P < 0.01, &&&P < 0.001, &&&&P < 0.0001 vs WT MCAO-operated mice.
